Microorganism

If you are in the field of microbial ecology, virology, microbiology, environmental sciences and biomedical research, we could be your perfect partner.

With a usual turnaround time of 4 to 6 weeks Macrogen offers total solutions for microbial profiling, gene markers or the whole microbial genome sequencing both at the de novo and re-sequencing level. Hybrid analysis of high depth of short reads from Illumina along with Pacbio’s long read approach would be a perfect solution for fungal genomes, while a throughput based Illumina runs on microbial samples would be a cost-effective approach.

	Metagenome Shotgun Sequencing	Amplicon Sequencing
		16s / 18s / ITS rDNA	Customised Amplicons
Platform	HiSeq2500 100bpPE	MiSeq3000 300bpPE
Sample Type	gDNA, Blood**, tissue, FFPE**
Library kit type	Truseq PCR free DNA kit (350bp insert) Truseq Nano DNA kit (350bp insert)	Truseq 16S amplicon protocol (Targeting V3-V4) same protocol will be used for 18S using different primer sets
Sample QC Requirement	Concentration: >10-20ng/ul Volume: >10-100ul Total Amount: 100ng-2ug Purity (A260/280 ratio): >1.7 DIN: 7.0 (Chromimum library only)	Concentration: >1ng/ul volume: >10ul Total Amount: 10ng
	For detailed sample requirement, please refer to following link: Download full list of ‘Sample QC Criteria’
Turnaround time**	4-6 weeks
Data Analysis	Whole Genome Shotgun Sequencing • De novo Assembly • BlastX • Gene Prediction • Functional Assignment • OTU Counting • Statistical Analysis	16R (or 18S) rRNA Sequencing • Global Alignment • Taxonomic Assignment • OTU Counting • Comparative Analysis

Recommended Coverage/ Reads length	10M reads	50-100K reads